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Title :An immunohistochemical study on the localization of type II collagen in the developing mouse mandibular condyle
Authors :Kenzaki, Keiichi
Tsuchikawa, Kohzo
Kuwahara, Toru
Publisher :Okajimas Folia Anatomica Japonica
Issue Date :Aug-2011
Journal Title :Okajimas Folia Anatomica Japonica
Volume :88
Issue :2
Start Page :49
End Page :55
ISSN :0030-154X
Abstract :The present chronological investigation assessed the distribution of type II collagen expression in the developing mouse mandibular condyle using immunohistochemical staining with respect to the anatomy of the anlage of the mandibular condyle, the histological characteristics of which were disclosed in our previous investigation. We analyzed fetuses, obtained by cross breeding of ICR strain mice, between 14.0 and 19.0 days post-conception (dpc) and pups on 1, 3, and 5 days post-natal (dpn) using immunohistochemical staining with 2 anti-type II collagen antibodies. The expression of type II collagen was first detected at 15.0 dpc in the lower part of the hypertrophic chondrocyte zone; thereafter, this type II collagen-positive layer was expanded and intensified (P_1 layer). At 17.0 dpc, we identified a type II collagen-negative layer ( N layer) around the P_1 layer and we also identified another newly formed type II collagen-positive layer (P_2 layer) on the outer surface of the N layer. The most typical and conspicuous 3-layered distribution was observed at 1 dpn; thereafter, there was a reduction in the intensity of expression, and with it, the demarcation between the layers was weakened by 5 dpn. The P_1 layer was derived from the central region of the core cell aggregate of the anlage of the mandibular condyle and participated in endochondral bone formation. The N layer was derived from the fringe of the core cell aggregate of the anlage, formed the bone collar at the side of the condyle by intramembranous bone formation, and showed a high level of proliferative activity at the vault. The P_2 layer was formed from the outgrowth of the N layer, and could be considered as the secondary cartilage. The intensive expression of type II collagen from 17.0 dpc to 3 dpn was detected in the fibrous sheath covering the condylar head, which is derived from the peripheral cell aggregate of the anlage. Since its expression in the fibrous sheath was not detected in the neighboring section in the absence of hyaluronidase digestion, some changes in the extracellular matrix of the fibrous sheath appear to participate in the generation of the lower joint space. The results of the present investigation indicate that further studies are required to fully characterize the development of the mouse mandibular condyle.
Keywords :Mandibular condylar cartilage
Type II collagen
Three-layered distribution
Secondary cartilage
Condylar anlage
Type Local :Journal Article
Language :eng
URI :http://hdl.handle.net/10623/32664
Relation :https://www.jstage.jst.go.jp/article/ofaj/88/2/88_2_49/_pdf
Appears in Collections:10 査読済論文 = Postprint

Please use this identifier to cite or link to this item: http://hdl.handle.net/10623/32664

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