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Title :3D observation of mouse embryo by micro-CT: Composition of trigeminal ganglion
Authors :Aoyagi, Hidekazu
Tsuchikawa, Kohzo
Iwasaki, Shin-ichi
Publisher :Springer
Issue Date :Feb-2010
Journal Title :Odontology : Official journal of the Society of the Nippon Dental University
Volume :98
Issue :1
Start Page :26
End Page :30
ISSN :1618-1247
Abstract :The purpose of this study was to demonstrate a micro-CT method for observations of the mouse embryo. Materials and methods: 13.0 days post-coitum (dpc) mouse embryos were fixed in 4% paraformaldehyde for 24h and stained en bloc by osmium teroxide overnight. The embryos were then embedded in paraffin using standard methods for 24h. Specimens were analyzed by micro-CT and image processing was performed. Results: Organs containing nervous and blood systems could be viewed as a result of different osmium-staining densities. In this paper, the trigeminal ganglion was imaged using 3D techniques. Conclusion: Observation of the embryo is possible by micro-CT with osmium tetroxide staining.
Type Local :Journal Article
Language :eng
URI :http://hdl.handle.net/10623/28382
Appears in Collections:10 査読済論文 = Postprint

Please use this identifier to cite or link to this item: http://hdl.handle.net/10623/28382

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